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Check the appearance of the cells under the microscope You may note the transfection complexes Incubate the chondrocytes at 37 C in a humidi ed atmosphere with 10% CO2 for 6 h As FuGENE 6-DNA complexes have not been reported to be harmful to the cells, it is usually not necessary to monitor the cells for signs of toxicity After 6 h, add 5 ml growth medium to the cell culture dish containing 10 ml of medium and hyaluronidase After 24 h, remove all medium Wash the cell layer once with 5 ml PBSA Trypsinize the cells i) Apply 2 ml trypsin-EDTA solution ii) Return the cells to the incubator for 15 45 min iii) After dissociation from the culture vessel, wash the cells once in 40 ml basal medium in a 50-ml Falcon tube Centrifuge at 2000 g for 5 min.

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, Naring, G, & van Der Staak, C (1994) Disease-speci c stressors in rheumatoid arthritis: Coping and well-being British Journal of Rheumatology, 33, 1067 1073 Ward, M M, Lotstein, D S, Bush, T M, Lambert, R E, van Vollen Hoven, R, & Newelt, C M (1999) Psychosocial.

iv) Determine the cell number by hemocytometer and cell viability by Trypan Blue exclusion v) Proceed to Protocol 53 to determine transfection ef ciency..

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Don t feel too intimidated by that message. Its only purpose is to convey a very common and well-accepted principle: If it ain t broke, don t fix it. Scroll down through to the Keybindings Editor, locate the entries for MythMusic, and edit the fields to the far right of the corresponding value you wish to modify. We personally find the default values to be perfectly well suited for general-purpose use, and therefore urge that no changes be made to their existing state. In the next segment we describe a more interesting place to begin hacking around with MythMusic: the Smart Playlist Editor.

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Like most desktop applications, Gmail actually allows you to mark a group of items without having to select each one individually (by mark, I mean to put a check in the checkbox next to an e-mail when you are presented with a list of e-mails). With Gmail, if you d like to select a group of consecutive messages without marking each one separately, you simply need to check the first one in the list, and then hold down the Shift key and check the last one you want to include in the group of marked messages the two e-mails you checked and all of the e-mails between them will now be marked. You can use the same method to unmark e-mails and to star or unstar them. Note, however, that this might not work in all browsers.

Consider the dynamics of g -mers, that is clusters containing g molecules. Assuming that cluster growth/shrinkage occurs by addition/removal of monomers only, the number concentration of g -mers Ng is described by the following differential equation [4]: Ng / t = (C g 1 Ng 1 + E g +1 Ng +1 ) (C g Ng + E g Ng ) (2.1)

(8.1)

Duplicating symbols Duplicating a symbol lets you use an existing symbol as a starting point for creating a new symbol. You can also use instances to create versions of the symbol with different appearances. See Creating instances on page 154.

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Unlike electrolytes, organic components have diverse chemical structures and possess quite different properties, both from each other and from electrolytes [18]. This necessitates activity model frameworks that are different to inorganic models. Indeed, because electrolytes and organics have different interactions in aqueous solutions, a large fraction of all activity coef cient models are applicable either for non-electrolyte solutions or for aqueous electrolyte solutions [114]. The breakthrough in the eld of g E models was achieved by Wilson (1964), who introduced the so-called local composition concept. This removed a major assumption

Figure 2-14

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